US PATENT SUBCLASS 435 / 471
.~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.)


Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440  DF  PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
471.~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.) {6}
472  DF  .~.~> The polynucleotide is encapsidated within a bacteriophage, bacteriophage coat, or transducing particle
473  DF  .~.~> The polynucleotide contains a transposon
474  DF  .~.~> The polynucleotide is a cosmid
475  DF  .~.~> The polynucleotide is unencapsidated bacteriophage or viral nucleic acid
476  DF  .~.~> The polynucleotide is a plasmid or episome {13}
490  DF  .~.~> The polynucleotide is an unbranched linear fragment


DEFINITION

Classification: 435/471

Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.):

(under subclass 440) Processes of inserting polynucleotide molecules into or rearranging genetic material within a microorganism such as bacteria, protozoa, bacteriophage, etc.

(1) Note. For purpose of this and indented subclasses, the term microorganism includes prokaryotes such as Escherichia

coli, Bacillus, Thermococcus, Halobacterium, and other bacteria; unicellular eukaryotes such as yeasts, molds, protozoans, and unicellular algae; and bacteriophage.

(2) Note. Insertion includes techniques such as transformation, transfection, transduction, conjugation, microinjection, particle-mediated transformation, lipofection, and infection.

(3) Note. Rearrangement includes processes which occur within a microbial cell such as transposition, gene duplication, and deletion or insertion of a polynucleotide segment from or into another polynucleotide segment, respectively.

(4) Note. Neither antisense oligonucleotides nor catalytic RNA molecules function as genetic material (i.e., do not encode genetic information) within cells. They act as agents which act upon molecules which do encode genetic information. Therefore processes of inserting an antisense oligonucleotide and/or catalytic RNA molecule into a cell are not included in this and indented subclasses.

SEE OR SEARCH THIS CLASS, SUBCLASS:

6, for processes of introducing nucleic acid into or rearrangement of nucleic acid within a microorganism wherein the final process step is isolating, selecting for, identifying, screening for, etc., the transformed cell. 243+, for a method of using antisense oligonucleotides or catalytic RNA to treat microbial cells.