US PATENT SUBCLASS 435 / 475
.~.~ The polynucleotide is unencapsidated bacteriophage or viral nucleic acid

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440  DF  PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
471  DF  .~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.) {6}
475.~.~ The polynucleotide is unencapsidated bacteriophage or viral nucleic acid

DEFINITION

Classification: 435/475

The polynucleotide is unencapsidated bacteriophage or viral nucleic acid:

(under subclass 471) Processes wherein the nucleic acid is that of a bacteriophage or virus which when inserted into the microbial cell is not surrounded by phage or viral coat protein.

(1) Note. Phage coat protein is a protein with a naturally occurring, mutated or recombinantly modified sequence, or a protein which is of a synthetically designed sequence, provided that said protein forms an organized array around the bacteriophage or viral nucleic acid and facilitates the independent entry of said nucleic acid into said cell.

(2) Note. The nucleic acid proper for this subclass is capable of infecting a microbial cell, i.e., is capable of directing the formation of infectious particles in said cell.

(3) Note. A phagemid or phasmid is a recombinant DNA vector that may act as a phage or as a plasmid, i.e., it is a hybrid between plasmid and filamentous phage cloning vehicles. If the phagemid is not encapsidated in phage coat, but encodes for phage coat protein, it is proper for the processes in this subclass. If the phagemid is not encapsidated and does not encode for phage coat protein, it is considered a plasmid and is not proper for this subclass.

(4) Note. Viruses of microorganisms and bacteriophages which never undergo a packaged, extracellular existence are included in this subclass.

(5) Note. Viroids and virusoids of microorganisms are proper for this subclass.

SEE OR SEARCH THIS CLASS, SUBCLASS: 472, for processes of introducing nucleic acid into a microorganism where the nucleic acid is encapsidated within a bacteriophage coat or transducing particle.

476+, for processes of introducing nucleic acid into a microorganism where the nucleic acid is plasmid or episome.