US PATENT SUBCLASS 435 / 476
.~.~ The polynucleotide is a plasmid or episome

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440  DF  PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
471  DF  .~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.) {6}
476.~.~ The polynucleotide is a plasmid or episome {13}
477  DF  .~.~.~> Plasmid or episome contains DNA targeting homologous recombination to bacteriophage, viral, or chromosomal DNA within a microorganism
478  DF  .~.~.~> Plasmid or episome contains at least part of a gene encoding a restriction endonuclease or modification enzyme
479  DF  .~.~.~> Plasmid or episome confers the ability to utilize directly a compound which a wild type microorganism is unable to utilize
480  DF  .~.~.~> Plasmid or episome contains at least part of a gene encoding a toxin or encoding for virulence or pathogenicity
481  DF  .~.~.~> Plasmid or episome contains a gene which complements a nutritional deficiency mutation
482  DF  .~.~.~> Plasmid or episome contains a gene which confers resistance to metal, silicon, selenium, or tellurium toxicity
483  DF  .~.~.~> Yeast is a host for the plasmid or episome
484  DF  .~.~.~> Mycelial fungus is a host for the plasmid or episome
485  DF  .~.~.~> Microorganism of the genus Bacillus is a host for the plasmid or episome
486  DF  .~.~.~> Microorganism of the genus Streptomyces is a host for the plasmid or episome
487  DF  .~.~.~> Microorganism of the genus Brevibacterium or the genus Corynebacterium is a host for the plasmid or episome
488  DF  .~.~.~> Microorganism of the genus Escherichia is a host for the plasmid or episome
489  DD  .~.~.~> Plural nonidentical plasmids are introduced into a host microorganism or culture thereof (e.g., plasmid is part of a library, etc.)

DEFINITION

Classification: 435/476

The polynucleotide is a plasmid or episome:

(under subclass 471) Processes wherein the nucleic acid is a plasmid or episome and therefore capable of autonomous replication in the microorganism cell.

(1) Note. The nucleic acid is either circular or linear in its replicating form and is circular, linear, or a linearized circle when introduced into the microbial cell.

(2) Note. An episome is a small, self-replicating molecule

of DNA that is separate from the main chromosome. It has the ability to move on and off the main chromosomal elements.

(3) Note. A phagemid or phasmid is a recombinant DNA vector that may act as a phage or as a plasmid, i.e., it is a hybrid between plasmid and filamentous phage cloning vehicles. If the phagemid is not encapsidated and does not encode for phage coat protein, it is considered a plasmid and proper for the processes of this and the indented subclasses.

SEE OR SEARCH THIS CLASS, SUBCLASS:

472, for processes of introducing nucleic acid into a microorganism where the nucleic acid is encapsidated within a bacteriophage coat or transducing particle.

475, for processes of introducing nucleic acid into a microorganism where the nucleic acid is unencapsidated bacteriophage or viral nucleic acid.