US PATENT SUBCLASS 435 / 472
.~.~ The polynucleotide is encapsidated within a bacteriophage, bacteriophage coat, or transducing particle

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440  DF  PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
471  DF  .~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.) {6}
472.~.~ The polynucleotide is encapsidated within a bacteriophage, bacteriophage coat, or transducing particle

DEFINITION

Classification: 435/472

The polynucleotide is encapsidated within a bacteriophage, bacteriophage coat, or transducing particle:

(under subclass 471) Processes wherein the nucleic acid which is to be introduced into a microbial cell is surrounded entirely or substantially by phage coat protein when brought into contact or proximity with said cell.

(1) Note. Phage coat protein is a protein with a naturally occurring, mutated, or recombinantly modified sequence, or a protein which is of a synthetically designed sequence, provided that said protein forms an organized array around the nucleic acid and facilitates independent entry of said nucleic acid into said cell.

(2) Note. A phagemid or phasmid is a recombinant DNA vector that may act as a phage or as a plasmid, i.e., it is a hybrid between plasmid and filamentous phage cloning vehicles. If the phagemid is encapsidated within a phage coat, it is proper for the processes in this subclass. If the phagemid

is not encapsidated and does not encode for phage coat protein, it is considered a plasmid and is not proper for this subclass.

(3) Note. A transducing particle is a DNA molecule surrounded by a complete bacteriophage protein coat, said particle facilitating independent entry of said DNA into a host microoorganism.

SEE OR SEARCH THIS CLASS, SUBCLASS:

475, for processes of introducing nucleic acid into a microorganism where the nucleic acid is unencapsidated bacteriophage or viral nucleic acid.

476+, for processes of introducing nucleic acid into a microorganism where the nucleic acid is plasmid or episome.