US PATENT SUBCLASS 435 / 455
.~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440  DF  PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
455.~ Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell {11}
456  DF  .~.~> The polynucleotide is encapsidated within a virus or viral coat {1}
458  DF  .~.~> The polynucleotide is coated with or encapsulated within a lipid containing material (e.g., liposome, etc.)
459  DF  .~.~> Involving particle-mediated transfection (i.e., biolistic transfection)
460  DF  .~.~> Involving laser treatment of the cell before or during transfection
461  DF  .~.~> Involving electroporation
462  DF  .~.~> Involving site-specific recombination (e.g., Cre-lox, etc.)
463  DF  .~.~> Involving general or homologous recombination (e.g., gene targeting, etc.)
464  DF  .~.~> Involving gene duplication within the cell (e.g., amplification, co-amplification, etc.)
465  DF  .~.~> Involving co-transfection
466  DF  .~.~> The polynucleotide is a shuttle vector or a transiently replicating hybrid vector
467  DF  .~.~> Introducing an oncogene to establish a cell line

DEFINITION

Classification: 435/455

Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell:

(under subclass 440) Processes of inserting polynucleotide molecules into or rearranging genetic material within an animal cell.

(1) Note. For purposes of this and the indented subclasses "animal cell" includes cells of organisms of the animal kingdom wherein said cells are not part of a tissue or an organ nor are they part of a multicellular organism during the process.

(2) Note. For purposes of this subclass, protozoans, also called protoctistans, are considered to be microorganisms and therefore methods of transforming them are classified elsewhere.

(3) Note. Insertion or introduction includes transfection, microinjection, particle-mediated transformation, lipofection, and infection.

(4) Note. Rearrangement includes processes which occur within a cell: transposition, gene duplication, and deletion or insertion of a polynucleotide segment from or into another polynucleotide segment, respectively.

(5) Note. Neither antisense oligonucleotides nor catalytic RNA molecules function as genetic material within cells. Therefore processes of inserting an antisense oligonucleotide and/or catalytic RNA molecule into a cell are excluded from this and the indented subclasses.

(6) Note. Processes of introducing nucleic acid into or rearrangement of nucleic acid within a cell wherein the final process step is to isolating, selecting for, identifying, screening for, etc. The transformed cells are considered to be test processes and are properly classified elsewhere.

SEE OR SEARCH THIS CLASS, SUBCLASS: 6, for methods of transforming cells with nucleic acid wherein the final step of the method involves measuring or

testing for the transformant (e.g., selecting, isolating, screening, identifying, etc).

375, for a method of using antisense oligonucleotides or catalytic RNA to treat animal cells in vitro.

471+, for methods of transforming protozoans.

SEE OR SEARCH CLASS

514, Drug, Bio-affecting and Body Treating Compositions,

44, for in vivo methods of treating animal cells within a multicellular organism with a polynucleotide.

800, Multicellular Living Organisms and Unmodified Parts Thereof and Related Processes,

21+, for methods of making nonhuman animals which may involve introduction of nucleic acid into or rearrangement of nucleic acid within the animal.