US PATENT SUBCLASS 800 / 278
METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART

Current as of: June, 1999
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800 /   HD   MULTICELLULAR LIVING ORGANISMS AND UNMODIFIED PARTS THEREOF AND RELATED PROCESSES

278METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART {14}
279  DF  .~> The polynucleotide confers pathogen or pest resistance {1}
281  DF  .~> The polynucleotide alters fat, fatty oil, ester-type wax, or fatty acid production in the plant
282  DF  .~> The polynucleotide alters pigment production in the plant
283  DF  .~> The polynucleotide alters ethylene production in the plant
284  DF  .~> The polynucleotide alters carbohydrate production in the plant
285  DF  .~> The polynucleotide encodes an inhibitory RNA molecule {1}
287  DF  .~> The polynucleotide contains a tissue, organ, or cell specific promoter
288  DF  .~> Nonplant protein is expressed from the polynucleotide
289  DF  .~> The polynucleotide confers resistance to heat or cold (e.g., chilling, etc.)
290  DF  .~> The polynucleotide alters plant part growth (e.g., stem or tuber length, etc.)
291  DF  .~> The polynucleotide comprises a transposon
292  DF  .~> Involving electroporation
293  DF  .~> Involving particle-mediated transfecion (e.g., biolistic, etc.)
294  DF  .~> Via Agrobacterium

DEFINITION

Classification: 800/278

METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART:

(under the class definition) Method for insertion of polynucleotide molecules into, or rearrangement of genetic material within a plant cell, wherein said cell is part of, or regenerated into, a plant or plant part.

(1) Note. The following glossary of terms is applicable to this and the indented subclasses:

A ntisense RNA and DNA

An approach for inhibiting functions of endogenous cellular genes which targets the gene's messenger RNA rather than the gene itself. An RNA or single-stranded DNA molecule that is complementary (antisense) to the mRNA of the target gene is introduced into cells. This antisense molecule can base-pair with the mRNA preventing translation of the mRNA into protein.

E nhancer

Element is a cis-acting sequence that increases the utilization of (some) eukaryotic promoters and can function in either orientation and in any location (upstream or downstream) relative to the promoter.

Exon

A continuous coding segment of a eukaryotic gene. Many eukaryotic genes are "split" and have exons interspersed with nonsense DNA called introns. Thus, it is a part of the gene which encodes protein. I ntron

DNA sequences in eukaryotes that lie within genes, but do not code for protein. In most instances, introns have no apparent function. Their presence "splits" the coding region of the gene into segments called exons. In the synthesis of messenger RNA, introns are copied into RNA, but they are removed by splicing, which restores the continuity of the coding sequence.

P romoter

Site on DNA where RNA polymerase binds and initiates transcription.

Regulator

Codes for an RNA or protein product whose function is to control the expression of other genes.

Structural gene

Codes for any RNA or protein product other than a regulator.

T erminator

A sequence of DNA, located at the end of the transcript, that causes RNA polymerase to terminate transcription.

Transcription unit The region between the sites of initiation and termination by RNA polymerase.

T ransposon

Mobile genetic element that can change its position within or between cellular genomes.