US PATENT SUBCLASS 435 / 91.2
.~.~.~.~.~.~ Acellular exponential or geometric amplification (e.g., PCR, etc.)

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

41  DF  MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION {26}
72  DF  .~ Preparing compound containing saccharide radical {13}
84  DF  .~.~ Preparing nitrogen-containing saccharide {1}
85  DF  .~.~.~ N-glycoside {3}
89  DF  .~.~.~.~ Nucleotide {3}
91.1  DF  .~.~.~.~.~ Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.) {4}
91.2.~.~.~.~.~.~ Acellular exponential or geometric amplification (e.g., PCR, etc.) {1}
91.21  DF  .~.~.~.~.~.~.~> Involving the making of multiple RNA copies

DEFINITION

Classification: 435/91.2

Acellular exponential or geometric amplification (e.g., PCR, etc.):

(under subclass 91.1) Processes which result in a geometric or exponential increase in the number of copies of a nucleotide sequence or sequences, rather than an arithmetic increase which occurs in the making of a single complementary copy of a nucleotide, wherein such processes take place without the involvement of viruses, eukaryotic cells, or prokaryotic cells.

(1) Note. Included in this subclass is the polymerase chain reaction (PCR). PCR is an in vitro DNA amplification system

that uses repeated cycles of oligonucleotide-directed DNA synthesis to selectively generate multiple copies of a specific DNA segment. It involves repeated cycles of (a) denaturation or strand displacement of the DNA, (b) annealing of two oligonucleotide primers that flank the DNA segment to be amplified, and (c) extension of the annealed primers with DNA polymerase.

(2) Note. Processes which result in an arithmetic increase in the number of copies of a nucleotide sequence, such as occurs in the making of a single complementary copy of a nucleotide sequence, are proper for subclasses 91.3+ if the nucleotide is a ribonucleotide and either subclass 91.1 or subclasses 91.5+, depending on the process details, if the nucleotide is other than a ribonucleotide.