US PATENT SUBCLASS 435 / 470-- .~.~ Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection

US PATENT SUBCLASS 435 / 470
.~.~ Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection

Current as of: June, 1999
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435 /	HD	CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

440	DF	PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION {6}
468	DF	.~ Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell {2}
470		.~.~ Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection

DEFINITION

Classification: 435/470

Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection:

(under subclass 468) Processes wherein the nucleic acid molecule is introduced into the plant cell by electroporation, particle, fiber or microprojectile mediated insertion, or injection.

(1) Note. Electroporation involves the use of an electric pulse of sufficient voltage and time to open temporary gaps in the cell membrane to permit a polynucleotide to enter the cell. Microprojectile insertion or injection involves the use of velocity or pressure to penetrate the plant cell wall and membrane in order to introduce the nucleic acid molecule into the cell. Particle or fiber mediated insertion involves using microscopic particles or fibers as carriers of the nucleic acid.