US PATENT SUBCLASS 435 / 378
.~ Method of detaching cells, digesting tissue or establishing a primary culture

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

325  DF  ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE {17}
378.~ Method of detaching cells, digesting tissue or establishing a primary culture {3}
379  DF  .~.~> Using mechanical means (e.g., trituration, etc.)
380  DF  .~.~> Releasing bound or adhered cell using protease
381  DF  .~.~> Digesting tissue with protease

DEFINITION

Classification: 435/378

Method of detaching cells, digesting tissue or establishing a primary culture:

(under subclass 325) Subject matter wherein animal cells are detached from a solid surface or matrix, or from one another, or a primary culture of animal cells is established.

(1) Note. Establishing a primary culture entails starting a culture of the organisms of choice from their original source, such as a tissue sample, blood, etc.