US PATENT SUBCLASS 435 / 188
.~ Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

183  DF  ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES {11}
188.~ Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate

DEFINITION

Classification: 435/188

Stabilizing an enzyme by forming a mixture, an adduct, or a composition, or formation of an adduct or enzyme conjugate:

(under subclass 183) Subject matter wherein (a) the enzyme is contacted with an extraneous material to impart to the enzyme a resistance to loss of activity, or (b) the enzyme is reacted with a nonenzymatic material to form a complex or a chemically modified enzymatic compound, e.g., conjugate, ligand, etc.

(1) Note. An enzyme conjugate, enzyme ligand, enzyme adduct for the purpose of this subclass are deemed to enhance enzyme stability.

(2) Note. In documents where it is unclear whether an enzyme joined to a chemical moiety is an immobilized enzyme or is an enzyme conjugate or adduct, the following factors should be considered. A. If the document states that the product is an enzyme conjugate, adduct, or ligand bound enzyme, placement is proper in subclass 188. B. If the ratio of non-enzyme moiety to enzyme is in the range of 0.01-100:1 placement would be indicated in subclass 188. A ratio of 1-40 nonenzyme moieties per enzyme indicates placement in subclass 188. C. If the molecular weight of the non-enzyme moiety is less than about 100,000, placement would be indicated in subclass 188. D. If the intended use of the enzyme containing product is a reagent in competitive assay, placement is indicated in subclass 188. If the use of the product is as a catalyst in the preparation of chemical compounds with recoverability (i.e., insolubility) an important consideration, placement as an immobililzed enzyme is indicated in subclasses 174+.