US PATENT SUBCLASS 435 / 181
.~.~.~ Attached to the carrier via a bridging agent

Current as of: June, 1999
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435 /   HD   CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY

174  DF  CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF {3}
177  DF  .~ Enzyme or microbial cell is immobilized on or in an organic carrier {2}
180  DF  .~.~ Carrier is synthetic polymer {2}
181.~.~.~ Attached to the carrier via a bridging agent

DEFINITION

Classification: 435/181

Attached to the carrier via a bridging agent:

(under subclass 180) Subject matter wherein the enzyme or microbial cell is bonded to the polymeric carrier through an intermediate compound which attaches to both the enzyme and the carrier.

(1) Note. The formation of the immobilized enzyme or cell may be in a stagewise manner with a reaction between the carrier and bridging agent being completed before the enzyme is added or in a process in which the carrier, bridging agent, and enzyme are present simultaneously in which case some care should be exercised in distinguishing the process of this subclass from mere entrapment. In general, if three separate entities, i.e., enzyme and two different chemical species are present simultaneously bonding through a bridging agent should be presumed.

(2) Note. A product or process classifiable in this subclass combines a polymeric carrier with a bridging agent to provide functional groups for enzyme attachment. It should be noted that similar functional groups can be provided by formation of a polymeric carrier by copolymerization of monomers one or more of which contain the desired functional group for enzyme attachment and that such would be provided for in subclass 180.